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Home >> Antibodies >> Cyclin B2

Cyclin B2

Mouse Monoclonal Antibody [Clone X29.2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Cyclin B2 Mouse Monoclonal Antibody (X29.2). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ccnb2; CycB2; Cyclin B2; G2 mitotic specific cyclin B2; HsT17299; MGC108931; MGC140694

Positive Control

HeLa cells. Human cervical tissue (IHC).

Known Applications & Suggested Dilutions

ELISA         More Details

ELISA (For coating, order antibody without BSA)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length Xenopus laevis Cyclin B2 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Mouse. Rat. Human. Xenopus laevis.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

51kDa

Specificity & Comments

In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mockel MM et al. Biol Open 6:463-470 (2017)

Bioinformatics

  •  Entrez Gene ID: 9133
  •  Gene Symbol: CCNB2
  •  SwissProt: O95067
  •  Chromosome Location: 15q22.2
  •  Unigene: 194698
NBT03059