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Home >> Antibodies >> Cyclin B1 (G2- & M-phase Cyclin)

Cyclin B1 (G2- & M-phase Cyclin)

Mouse Monoclonal Antibody [Clone V92.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Cyclin B1 Mouse Monoclonal Antibody (V92.1). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by Goat anti-mouse IgG-CF488 (Green).

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Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Positive Control

Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IP         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug/500ug protein) (precipitates active CDK1/cyclin B1 complexes)
Optimal dilution for a specific application should be determined.

Immunogen

Hamster Cyclin B1 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Hamster.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55-62kDa

Specificity & Comments

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Garner AP et al. Oncogene 21:8089-104 (2002)
  2. Minemoto Y et al. Exp Cell Res 262:37-48 (2001)

Bioinformatics

  •  Entrez Gene ID: 891
  •  Gene Symbol: CCNB1
  •  SwissProt: P14635
  •  Chromosome Location: 5q13.2
  •  Unigene: 23960
NBT00680