SDS-PAGE Analysis Purified Cyclin B1 Mouse Monoclonal Antibody (V92.1). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by Goat anti-mouse IgG-CF488 (Green).
Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.