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Home >> Antibodies >> CTLA4 / CD152 (Negative Regulator of T-Cells)

CTLA4 / CD152 (Negative Regulator of T-Cells)

Recombinant Rabbit Monoclonal Antibody [Clone CTLA4/6864R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human tonsil stained with CTLA4 Recombinant Rabbit Monoclonal Antibody (CTLA4/6864R). Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded human tonsil stained with CTLA4 Recombinant Rabbit Monoclonal Antibody (CTLA4/6864R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min

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SDS-PAGE Analysis Purified CTLA4 Recombinant Rabbit Monoclonal Antibody (CTLA4/6864R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ALPS5; CD152; Celiac disease 3 (CELIAC3); Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4); GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)

Positive Control

Jurkat or Raji cells. Lymph node or tonsil.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CTLA4 (exact sequence is proprietary)

Cellular Localization

Cell surface. Secreted.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

30-43kDa

Specificity & Comments

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a member of the CD28 superfamily and is a negative regulator of T cell-mediated immune responses. CTLA-4 expression is induced on the surface of T cells after CD28 binding and activation, and is constitutively expressed on T-regulatory cells, acting as an immune checkpoint inhibitor, downregulating T cell activity. CTLA-4 primarily inactivates T-cell activity by competing with the CD28 costimulatory molecule. CD28 and CTLA-4 share the identical ligands of CD80 and CD86 on antigenpresenting cells; and thus CTLA-4 competes with CD28 function in T-cell survival, proliferation, and recruitment. In particular, CTLA-4 down-modulates CD4+ helper T-cell activity and enhances Treg immunosuppressive functions. CTLA-4 has been shown to play a role in human diseases. CTLA-4 acts as a physiological brake on the activated immune system in order to maintain immune homeostasis. Several suppressive mechanisms for T-cell functions have been attributed to CTLA-4.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Peach, R.J., et al. 1995. Both extracellular immunoglobulin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28. J. Biol. Chem. 270: 21181-21187.

Bioinformatics

  •  Entrez Gene ID: 1493
  •  Gene Symbol: CTLA4
  •  SwissProt: P16410
  •  Chromosome Location: 2q33
  •  Unigene: 247824