Formalin-fixed, paraffin-embedded human skeletal muscle stained with Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4661). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human kidney cancer stained with Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4661). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4661).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into Î±, Î² and Î³ families, and the Î²- and Î³-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Î±-crystallins consist of three gene products, Î±A-, Î±B- and Î±C-crystallin, which are members of the small heat shock protein family (HSP 20). Î±-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, Î±-crystallins do not renature these proteins. Expression of Î±A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human Î±B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of Î±B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.