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Home >> Antibodies >> Complement 4d (C4d) (Acute Humoral Rejection Marker)

Complement 4d (C4d) (Acute Humoral Rejection Marker)

Mouse Monoclonal Antibody [Clone C4D204]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Kidney Transplant stained with Complement 4d Mouse Monoclonal Antibody (C4D204).

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Formalin-fixed, paraffin-embedded human Kidney Transplant stained with Complement 4d Mouse Monoclonal Antibody (C4D204).

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Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Complement 4d Mouse Monoclonal Antibody (C4D204).

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SDS-PAGE Analysis Purified Complement 4d Mouse Monoclonal Antibody (C4D204). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Acidic Complement C4; Basic C4; C4-1; C4a Anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4b; C4B1; C4B12; C4d fragment; C4F; C4S; Complement C4 Gamma Chain; Complement Component 4A; Complement Component 4B

Positive Control

Rejected Renal Transplant Tissue.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating use Ab at 1-2ug/ml order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Complement 4d protein

Cellular Localization

Intracytoplasmic vacuoles of endothelial cells and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

192kDa (predicted)

Specificity & Comments

This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Collins AB et. al. J Am Soc Nephrol. 1999;10(10):2208-14.
  2. Racusen LC et. al. Am J Transplant. 2003;3(6):708-14.

Bioinformatics

NBT00204