SDS-PAGE Analysis Purified Clathrin, HC Monoclonal Antibody (CHC/1432). Confirmation of Purity and Integrity of Antibody.
Confocal Immunofluorescence image of HeLa cells stained with Clathrin, HC Monoclonal Antibody (CHC/1432) followed by Goat anti-Mouse CF488 (green). Reddot is used to label the nuceli red.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.