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Home >> Antibodies >> CHD4

CHD4

Mouse Monoclonal Antibody [Clone 3F2/4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).

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SDS-PAGE Analysis Purified CHD4 Mouse Monoclonal Antibody (3F2/4). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).

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Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.

Product Details

Synonyms

ATP dependent helicase CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta

Positive Control

HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Cellular Localization

Nucleus and cytoplasm.

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

238kDa

Specificity & Comments

CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sparmann et al. 2013. EMBO J. 32(11):1598-612.

Bioinformatics

  •  Entrez Gene ID: 1108
  •  Gene Symbol: CHD4
  •  SwissProt: Q14839
  •  Chromosome Location: 12p13.31
  •  Unigene: 162233
NBT03077