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Home >> Antibodies >> CELA3B / ELA3B (Pancreatic Function Marker)

CELA3B / ELA3B (Pancreatic Function Marker)

Mouse Monoclonal Antibody [Clone CELA3B/1218]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot of Recombinant, Panc-28 and PANC1 cell lysates using CELA3B Monoclonal Antibody (CELA3B/1218).

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Western Blot Analysis of human PANC-1 cell lysate using CELA3B Monoclonal Antibody (CELA3B/1218)

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SDS-PAGE Analysis Purified CELA3B Monoclonal Antibody (CELA3B/1218). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E

Positive Control

Panc-28 or PANC1 cells. Pancreas.

Known Applications & Suggested Dilutions

ELISA         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~17kDa

Specificity & Comments

This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Shirasu, Y., et al. 1988. Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes. J. Biochem. 104:259-264.
  2. Shimada, S., et al. 2002. Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int. J. Mol. Med. 10: 599-603.

Bioinformatics

  •  Entrez Gene ID: 23436
  •  Gene Symbol: CELA3B
  •  SwissProt: P08861
  •  Chromosome Location: 1p36.12
  •  Unigene: 728752
NBT01218