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Home >> Antibodies >> CDw75 (B-Cell Marker)

CDw75 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone LN-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (LN-1).

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Surface staining of human lymphocytes with CDw75 Mouse Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

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Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with CDw75 Mouse Monoclonal Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N

Positive Control

HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
  2. Marder RJ et. al. Lab Invest 52: 497-504, 1985.

Bioinformatics

  •  Entrez Gene ID: 6480
  •  Gene Symbol: ST6GAL1
  •  SwissProt: P15907
  •  Chromosome Location: 3q27.3
  •  Unigene: 207459
NBT00055