2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Cdk1 / p34cdc2 Serine-Threonine Kinase

Cdk1 / p34cdc2 Serine-Threonine Kinase

Mouse Monoclonal Antibody [Clone A17.1.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Cdk1 Mouse Monoclonal Antibody (A17.1.1).

img

Western Blot Analysis of human HeLa cell lysate using Cdk1 Mouse Monoclonal Antibody (A17.1.1).

img

SDS-PAGE Analysis Purified Cdk1 Mouse Monoclonal Antibody (A17.1.1). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2

Positive Control

HeLa cells. Human tonsil (IHC).

Known Applications & Suggested Dilutions

IF, IP, Kinase Assay, Western Blot, IHC-P         More Details

Inhibits activation of p34cdc2 kinase by cyclins
Immunofluorescence (1-2ug/ml)
Immunoprecipitation
Kinase Assay
Western Blot
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

Cellular Localization

Nuclear and Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Guinea Pig. Woodchuck. Xenopus. Chicken.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Recognizes a 34kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Doussis-Anagnostopoulos IA, et. al. Histopathology, 1994, 24:335-40.

Bioinformatics

  •  Entrez Gene ID: 983
  •  Gene Symbol: CDC2
  •  SwissProt: P06493
  •  Chromosome Location: 10q21.2
  •  Unigene: 334562
NBT01083