Formalin-fixed, paraffin-embedded human Tonsil stained with Cdk1 Mouse Monoclonal Antibody (A17.1.1).
Western Blot Analysis of human HeLa cell lysate using Cdk1 Mouse Monoclonal Antibody (A17.1.1).
SDS-PAGE Analysis Purified Cdk1 Mouse Monoclonal Antibody (A17.1.1). Confirmation of Integrity and Purity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a 34kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.