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Home >> Antibodies >> Cdk1 / p34cdc2

Cdk1 / p34cdc2

Mouse Monoclonal Antibody [Clone POH-1; same as cdc2.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Cdk1 Mouse Monoclonal Antibody (POH-1).

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Western Blot Analysis of human HeLa cell lysate using Cdk1 Mouse Monoclonal Antibody (POH-1).

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SDS-PAGE Analysis Purified Cdk1 Mouse Monoclonal Antibody (POH-1). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2

Positive Control

HeLa or MCF-7 cells. Tonsil.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human p34cdc2 protein

Cellular Localization

Nuclear and Cytoplasmic

Species Reactivity

Human. Monkey. Cow. Mink. Does not react with Mouse, Rat, Xenopus, drosophila, S, pombe or S. cerevisiae.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lukas J et. al. European Journal of Biochemistry, 1992, 207(1):169-76.

Bioinformatics

  •  Entrez Gene ID: 983
  •  Gene Symbol: CDC2
  •  SwissProt: P06493
  •  Chromosome Location: 10q21.2
  •  Unigene: 334562
NBT00853