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Home >> Antibodies >> CD86 (Dendritic Cells Maturation Marker)

CD86 (Dendritic Cells Maturation Marker)

Recombinant Rabbit Monoclonal Antibody [Clone C86/2160R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Colon stained with CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).

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Immunofluorescent Staining of Raji cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-rabbit IgG conjugated with CF488 (green). Nuclei are stained with Red Dot.

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SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).

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Western Blot Analysis of Ramos cell lysate using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).

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Flow Cytometric Analysis of Ramos cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2

Positive Control

293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CD86 protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

70kDa

Specificity & Comments

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Engel P et. al. Blood. 1994;84(5):1402-7.

Bioinformatics

  •  Entrez Gene ID: 942
  •  Gene Symbol: CD86
  •  SwissProt: P4208
  •  Chromosome Location: 3q13.33
  •  Unigene: 171182
NBT02160