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Home >> Antibodies >> CD86 (Dendritic Cells Maturation Marker)

CD86 (Dendritic Cells Maturation Marker)

Mouse Monoclonal Antibody [Clone BU63]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

459.00

Formulation

Purified Ab Conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab Conjugated to PE

Price (USD)

459.00
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Flow Cytometry of human PBMCs using CD86 Monoclonal Antibody (BU63).

Product Details

Synonyms

Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2

Positive Control

293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Immunogen

ARH-77 (B-lymphoblastoid cell line)

Cellular Localization

Cell surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994;84(5):1402-7.
  2. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  3. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  4. Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994;180(5):1841-7.

Bioinformatics

  •  Entrez Gene ID: 942
  •  Gene Symbol: CD86
  •  SwissProt: P42081
  •  Chromosome Location: 3q13.33
  •  Unigene: 171182
NBT00193