Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Antibody (LN-2).
Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Mouse Monoclonal Antibody (LN-2).
Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of Human Raji cells using CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).