2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD73 (Immuno-Oncology Target)

CD73 (Immuno-Oncology Target)

Mouse Monoclonal Antibody [Clone NT5E/2646]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).

img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).

img

Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

img

Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).

img

SDS-PAGE Analysis Purified CD73 Mouse Monoclonal Antibody (NT5E/2646). Confirmation of Integrity and Purity of Antibody.

img

Western Blot Analysis of A431 and PC-3 cell lysate using Purified CD73 Mouse Monoclonal Antibody (NT5E/2646).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD73 Mouse Monoclonal Antibody (CD73/2646)

Product Details

Synonyms

5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase

Positive Control

A431, U87MG, PC-3 cells. Tonsil, Prostate or Lung Carcinoma.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human NT5E protein

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

71kDa

Specificity & Comments

CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yamashita, Y., et al. 1998. CD73 expression and Fyn-dependent signaling on murine lymphocytes. Eur. J. Immunol. 28: 2981-29

Bioinformatics

  •  Entrez Gene ID: 4907
  •  Gene Symbol: NT5E
  •  SwissProt: P21589
  •  Chromosome Location: 6q14.3
  •  Unigene: 153952
NBT02646