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Home >> Antibodies >> CD68 (Macrophage Marker)

CD68 (Macrophage Marker)

Recombinant Mouse Monoclonal Antibody [Clone rLAMP4/824]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4/824).

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4/824).

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Western Blot Analysis of human Spleen tissue lysate using CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4/824).

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SDS-PAGE Analysis Purified CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4/824). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence staining of U87MG cells using CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4/824) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot

Product Details

Synonyms

GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

Positive Control

U87MG, Tonsil, Lymph Node or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human LAMP4 / CD68 protein

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

110kDa

Specificity & Comments

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Holness, C.L. and Simmons, D.L. 1993. Blood 81: 1607-1613.

Bioinformatics

  •  Entrez Gene ID: 968
  •  Gene Symbol: CD68
  •  SwissProt: P34810
  •  Chromosome Location: 17p13.1
  •  Unigene: 647419
NBT03434