SDS-PAGE Analysis Purified CD64 Mouse Monoclonal Antibody (FCGR1A/7498). Confirmation of Integrity and Purity of Antibody.
IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Membrane stained using FCGR1A/7498 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Three different classes of IgG Fc receptors have been described: Fc Î³ RI (CD64), Fc Î³ RII (CD32) and Fc Î³ RIII (CD16). The low affinity receptors, CD64 and CD16, have a putative role in mediating humoral immune responses. CD64 is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-Î³ stimulation. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64 has been shown to associate with signal transducing subunit of the high affinity IgE receptor. Src family kinases Hck and Lyn show increased kinase activity and will coimmunoprecipitate with CD64 subsequent to receptor cross linking.