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Home >> Antibodies >> CD63 (Late Endosomes Marker)

CD63 (Late Endosomes Marker)

Mouse Monoclonal Antibody [Clone MX-49.129.5]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
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Flow Cytometry of human CD63 on MCF-7 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled CD63 Monoclonal Antibody (MX-49.129.5).

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IF staining of HeLa cells using CF488 labeled CD63 Monoclonal Antibody (MX-49.129.5) (Green). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI was used to stain the cell nucleus (blue).

Product Details

Synonyms

gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30

Positive Control

SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Full length CD63 of human origin

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26kDa (core protein); 30-60kDa (glycosylated)

Specificity & Comments

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985.
  2. AA Palmer et al., Pathology 17: 335-339, 1985.
  3. EC Hagen et al., Histopathology 10: 689-700, 1986.

Bioinformatics

  •  Entrez Gene ID: 967
  •  Gene Symbol: CD63
  •  SwissProt: P08962
  •  Chromosome Location: 12q13.2
  •  Unigene: 445570
NBT00525