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Home >> Antibodies >> CD59 / Complement Regulatory Protein / Protectin

CD59 / Complement Regulatory Protein / Protectin

Mouse Monoclonal Antibody [Clone 193-27]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

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Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

Product Details

Synonyms

20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein

Positive Control

Jurkat or Raji cells. Human lymphocytes. Human lymph node and tonsil.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Stimulated human leukocytes

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Baboon or Horse.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Shichishima T. et al. Diagnosis of paroxysmal nocturnal haemoglobinuria by phenotypic analysis of erythrocytes using two-colour flow cytometry with monoclonal antibodies to DAF and CD59/MACIF. Br J Haematol 1993, 85(2):378-386
  2. Navenot JM. et al. Investigation of the survival of paroxysmal nocturnal hemoglobinuria red cells through the immunophenotyping of reticulocytes. Transfusion 1998 38(4):337-342.
  3. Kishimoto T. et al., eds. Leukocyte Typing VI, p521-522 and p1157, Garland Publishing, Inc, New York and London, 1997.
  4. Murray EW and Robbins SM. Antibody cross-linking of the glycosylphosphatidylinositol-linked protein CD59 on hematopoietic cells induces signaling pathways resembling activation by complement. J Biol Chem 1998, 273(39):25279-25284.

Bioinformatics

NBT00182