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CD59 / Complement Regulatory Protein / Protectin Antibody [193-27]

In Stock
Catalog Number Formulation Size Price
966-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
966-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
966-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

Formalin-fixed, paraffin-embedded human bladder stained with CD59 Mouse Monoclonal Antibody (193-27).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Research Areas
Immunology, Complement System, Hematopoietic Stem Cells
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD59
Positive Control
Jurkat or Raji cells. Human lymphocytes. Human lymph node and tonsil.
Immunogen
Stimulated human leukocytes
Alternate Names
CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, 20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein

Database Links

Entrez Gene ID
966
SwissProt
P13987

Additional Information

Clone
193-27
Chromosome Location
11p13
Mol. Weight of Antigen
20kDa

Related Antibodies

View All CD59 Antibodies

Functions

  • Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
  • The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

Key References

  • Shichishima T. et al. Diagnosis of paroxysmal nocturnal haemoglobinuria by phenotypic analysis of erythrocytes using two-colour flow cytometry with monoclonal antibodies to DAF and CD59/MACIF. Br J Haematol 1993, 85(2):378-386
  • Navenot JM. et al. Investigation of the survival of paroxysmal nocturnal hemoglobinuria red cells through the immunophenotyping of reticulocytes. Transfusion 1998 38(4):337-342.
  • Kishimoto T. et al., eds. Leukocyte Typing VI, p521-522 and p1157, Garland Publishing, Inc, New York and London, 1997.
  • Murray EW and Robbins SM. Antibody cross-linking of the glycosylphosphatidylinositol-linked protein CD59 on hematopoietic cells induces signaling pathways resembling activation by complement. J Biol Chem 1998, 273(39):25279-25284.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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