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Home >> Antibodies >> CD54 / ICAM-1

CD54 / ICAM-1

Mouse Monoclonal Antibody [Clone W-CAM-1; same as Wehi-CAM-1 or 1H4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified CD54 Monoclonal Antibody (W-CAM-1). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).

Product Details

Synonyms

CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

Positive Control

Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IHC-P         More Details

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Raji Burkitt lymphoma cells

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

85-115kDa

Specificity & Comments

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Boyd AW et. al. Blood, 1989, 73(7):1896-903.
  2. Fecondo et al., Proc. Nat. Acad. Sci. 88(7), 28792882, (1991).
  3. Boyd AW et. al. Proceedings of the National Academy of Sciences, 1988, 85(9):3095
  4. Wawryk et al. J Clin Pathol 44, 497-501 (1991).

Bioinformatics

  •  Entrez Gene ID: 3383
  •  Gene Symbol: ICAM1
  •  SwissProt: P05362
  •  Chromosome Location: 19p13.3-p13.2
  •  Unigene: 643447
NBT00988