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Home >> Antibodies >> CD54 / ICAM-1

CD54 / ICAM-1

Mouse Monoclonal Antibody [Clone 15.2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analyis Purified CD54 Mouse Monoclonal Antibody (15.2). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

Positive Control

Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF, IHC-P         More Details

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human monocytes.

Cellular Localization

Cell Surface

Species Reactivity

Human. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

85-115kDa

Specificity & Comments

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Johnson, J.P., et al. Leucocyte Typing IV, Oxford Univ. Press, pp 681-683. (FC)
  2. Hromatka, BS et al. Biol Reprod. 2013 Jun 20;88(6):154. (IHC)
  3. Huegel R et al. J Invest Dermatol. 2007 Jan;127(1):65-74. (IC)

Bioinformatics

  •  Entrez Gene ID: 3383
  •  Gene Symbol: ICAM1
  •  SwissProt: P05362
  •  Chromosome Location: 19p13.3-p13.2
  •  Unigene: 643447
NBT02853