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Home >> Antibodies >> CD50 / ICAM3

CD50 / ICAM3

Mouse Monoclonal Antibody [Clone ICAM3/1019]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of HL-60 cell lysate using CD50 Monoclonal Antibody (ICAM3/1019)

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (ICAM3/1019)

Product Details

Synonyms

ICAMR; Intercellular adhesion molecule 3 (ICAM3)

Positive Control

HL-60 or THP-1 cells. Lymph node and tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human ICAM3 protein

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

110-160kDa

Specificity & Comments

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fawcett, J., Holness, C.L., Needham, L.A., Turley, H., Gatter, K.C., Mason, D.Y. and Simmons, D.L. 1992. Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes. Nature 360:481-484.

Bioinformatics

  •  Entrez Gene ID: 3385
  •  Gene Symbol: ICAM3
  •  SwissProt: P32942
  •  Chromosome Location: 19p13.2
  •  Unigene: 354563
NBT01019