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Home >> Antibodies >> CD47 / IAP (Integrin Associated Protein)

CD47 / IAP (Integrin Associated Protein)

Mouse Monoclonal Antibody [Clone B6H12.2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Immunofluorescent staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Redot (red).

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Flow Cytometric Analysis of live Jurkat cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (B6H12.2). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescent image of live Jurkat cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-mouse IgG-CF488 (green).

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Immunofluorescent image of PFA-fixed Jurkat cells.CD47 Mouse Monoclonal Antibody (B6H12.2) followed bygoat anti-mouse IgG-CF488 (green).

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Flow Cytometric Analysis of PFA-fixed Jurkat cells.CD47 Mouse Monoclonal Antibody (B6H12.2) followed bygoat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen

Positive Control

Jurkat, MCF-7 or OVCA3 cells. Human placenta, brain or ovarian tumors.

Known Applications & Suggested Dilutions

Func., Western Blot, Flow Cyt, IF         More Details

Functional Studies (Order Ab without Azide)
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Intact CD47 Purified from placenta

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Edris B et. al. Proc Natl Acad Sci USA 109:6656-61 (2012).
  2. Willingham SB et. al. Proc Natl Acad Sci USA 109:6662-7 (2012).

Bioinformatics

  •  Entrez Gene ID: 961
  •  Gene Symbol: CD47
  •  SwissProt: Q08722
  •  Chromosome Location: 3q13.12
  •  Unigene: 446414
NBT00437