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Home >> Antibodies >> CD47 / IAP (Integrin Associated Protein)

CD47 / IAP (Integrin Associated Protein)

Mouse Monoclonal Antibody [Clone CD47/2937]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/2937).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/2937).

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SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/2937). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of U87, THP-1 cell lysate using CD47 Mouse Monoclonal Antibody (IAP/2937).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/2937)

Product Details

Synonyms

Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen

Positive Control

MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Edris B et. al. Proc Natl Acad Sci USA 109:6656-61 (2012).
  2. Willingham SB et. al. Proc Natl Acad Sci USA 109:6662-7 (2012).

Bioinformatics

  •  Entrez Gene ID: 961
  •  Gene Symbol: CD47
  •  SwissProt: Q08722
  •  Chromosome Location: 3q13.12
  •  Unigene: 446414
NBT02937