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Home >> Antibodies >> CD45RO (T-Cell Marker)

CD45RO (T-Cell Marker)

Mouse Monoclonal Antibody [Clone T200/797]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Mouse Monoclonal Antibody (T200/797).

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SDS-PAGE Analysis CD45RO Mouse Monoclonal Antibody (T200/797). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Mouse Monoclonal Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human T-lymphocytes

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

180-185kDa

Specificity & Comments

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. West, K.P., et al. 1986. The demonstration of B cell, T cell and myeloid antigens in paraffin sections. J. Pathol. 150: 89-101.

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514
NBT00797