Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Mouse Monoclonal Antibody (190-2F2.5) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45 with CD45 Mouse Monoclonal Antibody (190-2F2.5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
SDS-PAGE Analysis Purified CD45 Mouse Monoclonal Antibody (190-2F2.5). Confirmation of Integrity and Purity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.