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Home >> Antibodies >> CD45RO (T-Cell Marker)

CD45RO (T-Cell Marker)

Mouse Monoclonal Antibody [Clone 190-2F2.5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Mouse Monoclonal Antibody (190-2F2.5) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45 with CD45 Mouse Monoclonal Antibody (190-2F2.5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

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SDS-PAGE Analysis Purified CD45 Mouse Monoclonal Antibody (190-2F2.5). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Lentillectin Purified glyco proteins obtained from HuPBL and from a CLL (B cell).

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

180-185kDa

Specificity & Comments

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sparrow RL et al. A function for human T200 in natural killer (NK) cytolysis. Transplantation 36, 166171 (1983).

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514
NBT01081