Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (SPM568).
Flow Cytometric Analysis of Jurkat cells using CD45RA Monoclonal Antibody (SPM568) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot Analysis of human spleen tissue lysates using CD45RA Monoclonal Antibody (SPM568).
Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45RA MAb (SPM568) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).