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Home >> Antibodies >> CD45 / LCA (B-Cell Marker)

CD45 / LCA (B-Cell Marker)

Mouse Monoclonal Antibody [Clone PTPRC/1461]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (PTPRC/1461).

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Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45 Monoclonal Antibody (PTPRC/1461) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

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Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Monoclonal Antibody (PTPRC/1461) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

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SDS-PAGE Analysis Purified CD45 Monoclonal Antibody (PTPRC/1461). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

Jurkat, Ramos, U-698 or GA-10 cells. Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human B-lymphocytes

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  2. Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514
NBT01461