Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (PTPRC/1461).
Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45 Monoclonal Antibody (PTPRC/1461) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Monoclonal Antibody (PTPRC/1461) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
SDS-PAGE Analysis Purified CD45 Monoclonal Antibody (PTPRC/1461). Confirmation of Integrity and Purity of Antibody
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.