Home >> Antibodies >> CD45 / LCA (Leucocyte Marker)

CD45 / LCA (Leucocyte Marker)

Mouse Monoclonal Antibody [Clone 2B11 + PD7/26]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Mouse Monoclonal Antibody (2B11+PD7/26) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Western Blot Analysis of human Spleen tissue lysates using CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Flow Cytometric Analysis of live Jurkat cells unstained (grey) and using CD45 Mouse Monoclonal Antibody (2B11+PD7/26) conjugated with CF680 (purple).

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD45 monoclonal antibody (2B11+PD7/26) (green).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Mouse Monoclonal Antibody (2B11+PD7/26) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Western Blot Analysis of human Spleen tissue lysates using CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Flow Cytometric Analysis of live Jurkat cells unstained (grey) and using CD45 Mouse Monoclonal Antibody (2B11+PD7/26) conjugated with CF680 (purple).

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD45 monoclonal antibody (2B11+PD7/26) (green).

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

PBMCs, Jurkat, Ramos, U-698 or GA-10 cells. Tonsil and Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Dog.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.