Home >> Antibodies >> CD44 / HCAM Std. (Cancer Stem Cell Marker)

CD44 / HCAM Std. (Cancer Stem Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rHCAM/918]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human cervix stained with CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

Formalin-fixed, paraffin-embedded human tonsil stained with CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

SDS-PAGE Analysis Purified CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of HeLa cell lysate using CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

Formalin-fixed, paraffin-embedded human cervix stained with CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

Formalin-fixed, paraffin-embedded human tonsil stained with CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

SDS-PAGE Analysis Purified CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of HeLa cell lysate using CD44 Recombinant Mouse Monoclonal Antibody (rHCAM/918).

Product Details

Synonyms

LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group

Positive Control

HeLa cells (FACS). HeLa cell lysate (WB). Paracortex in tonsil or lymph node (IHC).

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF, Western Blot, IHC-P More Details

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD44 protein

Cellular Localization

Cell Surface

Species Reactivity

Human, Baboon, and Green Monkey.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

80-95kDa

Specificity & Comments

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.