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Home >> Antibodies >> CD44 / HCAM Std. (Cancer Stem Cell Marker)

CD44 / HCAM Std. (Cancer Stem Cell Marker)

Mouse Monoclonal Antibody [Clone HCAM/918]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD44 Mouse Monoclonal Antibody (HCAM/918).

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Western Blot Analysis of HeLa cell lysate using CD44 Mouse Monoclonal Antibody (HCAM/918).

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Western Blot Analysis of DU145 cell lysate using CD44 Mouse Monoclonal Antibody (HCAM/918).

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Confocal immunofluorescence image of HeLa cells using CD44 Mouse Monoclonal Antibody (HCAM/918) in Green (CF488) and Reddot is used to label the nuclei Red.

Product Details

Synonyms

LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)

Positive Control

HeLa cells. Paracortex in tonsil or lymph node.

Known Applications & Suggested Dilutions

Func., Western Blot, Flow Cyt, IF, IHC-P         More Details

Functional Studies (Order Ab without BSA & Azide)
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Stimulated human leukocytes

Cellular Localization

Cell Surface

Species Reactivity

Human. Baboon. Green Monkey.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

80-95kDa

Specificity & Comments

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.

Bioinformatics

  •  Entrez Gene ID: 960
  •  Gene Symbol: CD44
  •  SwissProt: P16070
  •  Chromosome Location: 11p13
  •  Unigene: 502328
NBT00918