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Home >> Antibodies >> CD43 (T-Cell Marker)

CD43 (T-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rSPN/839]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lymph Node stained with CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839).

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SDS-PAGE Analysis Purified CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of K562 cell lysate using CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839).

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Flow Cytometric Analysis of PFA-fixed K562 cells using CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

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Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Product Details

Synonyms

Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Positive Control

K562 cells. Paracortex in a tonsil or a reactive lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD43 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

95, 115, or 135kDa

Specificity & Comments

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called b as opposed to a for rSPN/839. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stross WP, et. al. Journal of Clinical Pathology, 1989, 42(9):953-61.

Bioinformatics

  •  Entrez Gene ID: 6693
  •  Gene Symbol: SPN
  •  SwissProt: P16150
  •  Chromosome Location: 16p11.2
  •  Unigene: 632188
NBT02220