2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD43 (T-Cell Marker)

CD43 (T-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rSPN/1094]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094).

img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094).

img

SDS-PAGE Analysis Purified CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094). Confirmation of Purity and Integrity of Antibody.

img

Western Blot Analysis of K562 cell lysate using CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094).

img

Immunofluorescent staining of K562 cells. CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094). The nuclear counterstain is Reddot (Red)

img

Flow Cytometric Analysis of Human K562 cells. CD43 Mouse Recombinant Monoclonal Antibody (rSPN/1094) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Synonyms

Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Positive Control

K562 cells, Paracortex in a tonsil or a reactive lymph node.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD43 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

95, 115, or 135kDa

Specificity & Comments

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baeckstrom, D., et al. 1995. Expression of the leukocyte-associated sialo-glycoprotein CD43 by a colon carcinoma cell line. J. Biol. Chem. 270: 13688-13692.

Bioinformatics

  •  Entrez Gene ID: 6693
  •  Gene Symbol: SPN
  •  SwissProt: P16150
  •  Chromosome Location: 16p11.2
  •  Unigene: 632188
NBT01858