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Home >> Antibodies >> CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)

CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)

Mouse Monoclonal Antibody [Clone CD40LG/2761]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Spleen stained with CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761).

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Formalin-fixed, paraffin-embedded human Spleen stained with CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761)

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Immunofluorescent staining of paraformaldehyde-fixed Jurkat cells using CD40L Mouse Monoclonal Antibody (CD40LG/2761) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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Flow Cytometric Analysis of Jurkat cells using CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761).

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SDS-PAGE Analysis Purified CD40L Mouse Monoclonal Antibody (CD40LG/2761). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD40-Ligand Mouse Monoclonal Antibody (CD40LG/2761)

Product Details

Synonyms

CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell activating molecule; T BAM; T-cell antigen Gp39; TNF-related activation protein; TrAP; Tumor necrosis factor ligand superfamily member 5 (TNFSF5)

Positive Control

Jurkat cells. Tonsil, Spleen or Thymus.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)

Cellular Localization

Cell Surface and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Howland, K. et al. J. Immunol.164:4465-4470.

Bioinformatics

  •  Entrez Gene ID: 959
  •  Gene Symbol: CD40LG
  •  SwissProt: P29965
  •  Chromosome Location: Xq26.3
  •  Unigene: 592244
NBT02761