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Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Rabbit Polyclonal Antibody

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western blot of CD3e in human Jurkat cells (1) absence and (2) presence of immunizing peptide. (3) Mouse thymus probed with CD3e Rabbit Polyclonal Antibody.

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Western Blot of Jurkat cell lysate using CD3e Rabbit Polyclonal Antibody.

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SDS-PAGE Analysis Purified CD3e Rabbit Polyclonal Antibody Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Positive Control

Jurkat cells. Tonsil and lymph node.

Known Applications & Suggested Dilutions

Western Blot         More Details

Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human. Predicted to react with Ape, Orangutan, Gibbon, Chimpanzee, Monkey, Elephant, Horse, Cow, Sheep, Pig, Dog, Cat, Rabbit, Rat, Mouse, Guinea Pig, Hamster, Opossum, Frog, Chicken.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

20kDa

Specificity & Comments

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Related Products



200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
  2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

Bioinformatics

  •  Entrez Gene ID: 916
  •  Gene Symbol: CD3E
  •  SwissProt: P07766
  •  Chromosome Location: 11q23.3
  •  Unigene: 3003
NBT00385