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Home >> Antibodies >> CD34 (Hematopoietic Stem Cell & Endothelial Marker)

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rHPCA1/8573]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human uterus stained with CD34 Recombinant Mouse Monoclonal Antibody (rHPCA1/8573). HIER: Tris/EDTA, pH9.0, 45min. 2░: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human stomach stained with CD34 Recombinant Mouse Monoclonal Antibody (rHPCA1/8573). HIER: Tris/EDTA, pH9.0, 45min. 2░: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human colon cancer stained with CD34 Recombinant Mouse Monoclonal Antibody (rHPCA1/8573). HIER: Tris/EDTA, pH9.0, 45min. 2░: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Positive Control

Human tonsil or angiosarcoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa100-300) of human HPCA1 protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, lambda

Mol. Weight of Antigen

90-110kDa

Specificity & Comments

This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into áthree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. áCD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Ramani P; Bradley NJ; Fletcher CD. Histopathology, 1990, 17:237-42.

Bioinformatics

  •  Entrez Gene ID: 947
  •  Gene Symbol: CD34
  •  SwissProt: P28906
  •  Chromosome Location: 1q32.2
  •  Unigene: 374990