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Home >> Antibodies >> CD34 (Hematopoietic Stem Cell & Endothelial Marker)

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Mouse Monoclonal Antibody [Clone ICO-115]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to PE

Price (USD)

429.00
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Flow cytometry of KG-1 cells using CD34 Monoclonal Antibody (ICO-115) (red) and isotype control Ab (green).

Product Details

Synonyms

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Positive Control

KG-1 cells. Human tonsil or angiosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Immunogen

Blast cells of a chronic myeloid leukemia patient

Cellular Localization

Cell surface

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

90-110kDa

Specificity & Comments

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Frolova, E., Baryshnikov A.Y., Frenkel M. et al., Monoclonal antibody ICO-115 to human CD34 antigen. Herald of the Cancer Research Center of RAMS. Suppl, p. 10-12, 1994.
  2. Sato T et al. Blood 94:2548-2554 (1999).
  3. Felshow DM et al. Blood 97:3768-3775 (2001).
  4. Baryshnikov A, and Tonevitsky A, Monoclonal antibodies in laboratory and clinic. Thesis p212, 1997.
  5. Kishimoto et al., Leucocyte typing Gariand Publishing. 1997.

Bioinformatics

  •  Entrez Gene ID: 947
  •  Gene Symbol: CD34
  •  SwissProt: P28906
  •  Chromosome Location: 1q32.2
  •  Unigene: 374990
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