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Home >> Antibodies >> CD34 (Hematopoietic Stem Cell & Endothelial Marker)

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone HPCA1/8353R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human uterus stained with CD34 Rabbit Recombinant Monoclonal Antibody (HPCA1/8353R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human uterus stained with CD34 Rabbit Recombinant Monoclonal Antibody (HPCA1/8353R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Positive Control

Jurkat or KG-1 cells. Human tonsil or angiosarcoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD34 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

41kDa

Specificity & Comments

CD34 (also named myeloid progenitor cell antigen) is a heavily glycosylated type I transmembrane protein. There are two forms of the CD34 protein, resulting from alternative splicing. The functions of CD34 is largely unknown, but recent evidence suggests a role for CD34 in cell adhesion and inhibition of hematopoiesis. It is suggested that CD34 is a signaling molecule involved in maintenance of a phenotypically plastic state in undifferentiated cells. CD34 is found in most endothelia, expressed on the luminal surface and membrane processes interdigitating between endothelial cells, but is absent from large veins and arteries. CD34 is furthermore expressed in fibroblast-like dendritic cells in, e.g., portal tracts of the liver, Peyer's patches, and in healing wounds. In smooth muscle cells, a variable CD34 staining is found. In tumor tissues, CD34 is detected in myeloid blasts in myelodysplastic syndrome and acute myeloid leukemia in most cases as well as lymphoblasts in most cases of B-acute lymphoblastic leukemia. Mature B- and T-cell lymphomas and leukemias are CD34 negative. The majority of vascular tumors, including hemangiosarcoma and Kaposi sarcoma are CD34positive. In a panel, CD34 staining is useful for the classification of myeloid and lymphoid neoplasms as well as spindle cell neoplasms (particularly identification of gastrointestinal stromal tumor and hemangiosarcoma).

Related Products



200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Felshow DM et al. Blood 97:3768-3775 (2001).
  2. Sato T et al. Blood 94:2548-2554 (1999).

Bioinformatics

  •  Entrez Gene ID: 947
  •  Gene Symbol: CD34
  •  SwissProt: P28906Human
  •  Chromosome Location: 1q32.2
  •  Unigene: 374990