Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Rabbit Recombinant Monoclonal Antibody (HPCA1/1806R).
SDS-PAGE Analysis of Purified CD34 Rabbit Recombinant Monoclonal Antibody (HPCA1/1806R).
Flow Cytometric Analysis of human Jurkat cells using CD34 Rabbit Recombinant Monoclonal Antibody (HPCA1/1806R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.