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Home >> Antibodies >> CD33 / SIGLEC3 (Myeloid Cell Surface Antigen)

CD33 / SIGLEC3 (Myeloid Cell Surface Antigen)

Mouse Monoclonal Antibody [Clone WM53]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab Conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab Conjugated to CF488

Price (USD)

429.00

Product Details

Synonyms

gp67; My9; Myeloid cell surface antigen CD33; p67; Sialic acid-binding Ig-like lectin 3 (SIGLEC3)

Positive Control

Human dendritic and mast cells. Human lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry: 5ul per test per one million cells (or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Immunogen

Human AML cells

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 1987;5(4):215-25.
  2. Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988;69(2):163-71.

Bioinformatics

  •  Entrez Gene ID: 945
  •  Gene Symbol: CD33
  •  SwissProt: P20138
  •  Chromosome Location: 19q13.33
  •  Unigene: 83731
NBT00166