SDS-PAGE Analysis Purified CD33 Mouse Monoclonal Antibody (SIGLEC3/3600). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human spleen stained with CD33 Mouse Monoclonal Antibody (SIGLEC3/3600).
Formalin-fixed, paraffin-embedded human urothelial carcinoma stained with CD33 Mouse Monoclonal Antibody (SIGLEC3/3600).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with CD33 Mouse Monoclonal Antibody (SIGLEC3/3600).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD33 Mouse Monoclonal Antibody (SIGLEC3/3600).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.