2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD32 (Fc Gamma RIIa)

CD32 (Fc Gamma RIIa)

Mouse Monoclonal Antibody [Clone FCGR2A/479]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

SDS-PAGE Analysis Purified CD32 Mouse Monoclonal Antibody (FCGR2A/479). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD32A; Fc fragment of IgG low affinity IIa receptor; Fc gamma RIIa; FCGR2A; FcgRII; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17

Positive Control

K-562 cells. Lymph node, tonsil and placenta.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human FCGR2A protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

This MAb binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Maria-Concetta Veri, et al. Jul 2007; 121(3): 392-404.
  2. Jutta Horejs-Hoeck, et al. Immunology. Jul 2005; 115(3): 407-415.

Bioinformatics

  •  Entrez Gene ID: 2212
  •  Gene Symbol: FCGR2A
  •  SwissProt: P12318
  •  Chromosome Location: 1q23
  •  Unigene: 352642
NBT00479