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Home >> Antibodies >> CD32 (Fc Gamma RIIa)

CD32 (Fc Gamma RIIa)

Mouse Monoclonal Antibody [Clone 7.3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified CD32 Mouse Monoclonal Antibody (7.30). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD32A; Fc fragment of IgG low affinity IIa receptor; Fc gamma RIIa; Fc-gamma RII-a; Fc-gamma-RIIa; FCGR2A; FcgRII; Fcr-2; FcRII-a; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17

Positive Control

K-562 cells. Lymph node, tonsil and placenta.

Known Applications & Suggested Dilutions

Func., IF         More Details

Functional Studies (Order Ab without BSA & Azide)Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

K562 and FcgRII+L cells

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ierino et al., J. Immunol, 150: 17941803 (1993).

Bioinformatics

  •  Entrez Gene ID: 2212
  •  Gene Symbol: FCGR2A
  •  SwissProt: P12318
  •  Chromosome Location: 1q23
  •  Unigene: 352642
NBT01078