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Home >> Antibodies >> CD31 / PECAM-1 (Endothelial Cell Marker)

CD31 / PECAM-1 (Endothelial Cell Marker)

Mouse Monoclonal Antibody [Clone C31.7]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31 Mouse Monoclonal Antibody (C31.7).

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Flow Cytometric Analysis of human CD31 on Jurkat cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31 Mouse Monoclonal Antibody (C31.7).

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Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31 Mouse Monoclonal Antibody (C31.7).

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Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.7).

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Western Blot of CD31 in THP-1 lysate using CD31 Mouse Monoclonal Antibody (C31.7).

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SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (C31.7). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of paraformaldehyde-fixed Jurat cells using CD31 Mouse Monoclonal Antibody (158-2B3) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA

Positive Control

Jurkat cells. Tonsil or Angiosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CD31 protein

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Cynomolgus Monkey. Rabbit. Weakly reacts with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~100kDa (endothelium) and ~130kDa (platelets)

Specificity & Comments

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).

Bioinformatics

  •  Entrez Gene ID: 5175
  •  Gene Symbol: PECAM1
  •  SwissProt: P16284
  •  Chromosome Location: 17q23.3
  •  Unigene: 376675, 514412
NBT00014