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Home >> Antibodies >> CD31 / PECAM-1 (Endothelial Cell Marker)

CD31 / PECAM-1 (Endothelial Cell Marker)

Mouse Monoclonal Antibody [Clone 1A10]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Culture Supernatant
Culture Supernatant

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Culture Supernatant
Culture Supernatant

Price (USD)

199.00
429
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD31 Monoclonal Antibody (1A10)

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Western Blot Analysis of human THP-1 cell lysate using CD31 Monoclonal Antibody (1A10)

Product Details

Synonyms

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

Positive Control

THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)

Cellular Localization

Cell surface and cytoplasm of endothelial cells

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~100kDa (endothelium) and ~130kDa (platelets)'

Specificity & Comments

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Related Products



Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Key References

  1. Stockinger H, Gadd S J, Eher R, et al. Molecular characterization and functional analysis of the leukocyte surface protein CD31. Journal of Immunology. 145 (11): 3889-3897 (1990).
  2. Stockinger H, Schreiber W, Majdic O, et al. Phenotype of human T cells expressing CD31, a molecule of the immunoglobulin supergene family. Immunology. 75 (1): 53-58 (1992)

Bioinformatics

  •  Entrez Gene ID: 5175
  •  Gene Symbol: PECAM1
  •  SwissProt: P16284
  •  Chromosome Location: 17q23.3
  •  Unigene: 376675, 514412
NBT00846