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Home >> Antibodies >> CD31 / PECAM-1 (Endothelial Cell Marker)

CD31 / PECAM-1 (Endothelial Cell Marker)

Mouse Monoclonal Antibody [Clone 158-2B3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (158-2B3) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

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SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (158-2B3). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescent staining of PFA-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (158-2B3) followed by goat anti-Mouse IgG conjugated with CF488 (green). Nuclei are labeled with RedDot (red).

Product Details

Synonyms

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

Positive Control

Jurkat cells. Tonsil or Angiosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Stimulated human leukocytes (Workshop VI)

Cellular Localization

Cell surface and cytoplasm of endothelial cells

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~100kDa (endothelium) and ~130kDa (platelets)'

Specificity & Comments

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. The Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens, Kobe, Japan, 1996, in press (Garland Publishing Inc., London).

Bioinformatics

  •  Entrez Gene ID: 5175
  •  Gene Symbol: PECAM1
  •  SwissProt: P16284
  •  Chromosome Location: 17q23.3
  •  Unigene: 376675, 514412
NBT00352