2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD28

CD28

Mouse Monoclonal Antibody [Clone 204.12]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to labelnuclei (red).

img

Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

img

SDS-PAGE Analysis Purified CD28 Mouse Monoclonal Antibody (204.12). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

T-cell-specific surface glycoprotein CD28; Tp44

Positive Control

Jurkat cells. Human lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml) Functional Studies (Order Ab without BSA & Azide)
Optimal dilution for a specific application should be determined.

Immunogen

Stimulated human leucocytes

Cellular Localization

Cell Surface

Species Reactivity

Human. Pig. Cow. Sheep. Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

44kDa (monomer); 88kDa (dimer)

Specificity & Comments

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. In Leucocyte typing Workshop VI. Sandilands, G. et al. Clinical and Experimental Immunology, 162(3), 516-27 (2010).

Bioinformatics

  •  Entrez Gene ID: 940
  •  Gene Symbol: CD28
  •  SwissProt: P10747
  •  Chromosome Location: 2q33.2
  •  Unigene: 591629
NBT00164