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Home >> Antibodies >> CD22 / BL-CAM (B-Cell Marker)

CD22 / BL-CAM (B-Cell Marker)

Mouse Monoclonal Antibody [Clone MYG13]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (MYG13). Confirmation of Integrity and Purity of Antibody.

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Flow Cytometric Analysis of Ramos cells using CD22 Mouse Monoclonal Antibody (MYG13) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14

Positive Control

Ramos, Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human tonsil and lymphocytes.

Known Applications & Suggested Dilutions

Flow Cyt         More Details

Flow Cytometry (1-2ug/million cells)
Optimal dilution for a specific application should be determined.

Immunogen

Human Raji cells

Cellular Localization

Cell Surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

130-140kDa

Specificity & Comments

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schlossman SF et al. eds. Leukocyte Typing V, p523-503, Oxford University Press, Oxford, 1989.
  2. Knapp, W et al. eds Leukocyte Typing IV, p190-192, Oxford University Press, Oxford, 1989
  3. Tedder TF et al. CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol 15:481-504.
  4. Cyster JG and Goodnow CC. Tuning antigen receptor signaling by CD22: integrating cues from antigens and the microenvironment. Immunity 1997,6:509-517.
  5. Tuscano JM et al. Involvement of p72syk kinase, p53/56lyn kinase and phosphatidyl inositol-3 kinase in signal transduction via the human B lymphocyte antigen CD22. Eur J Immunol 1996, 26:1246-1252.
  6. Sato S et al. CD22 negatively and positively regulates signal transduction through the B lymphocyte antigen receptor. Semin Immunol 1998, 10:287-297.

Bioinformatics

  •  Entrez Gene ID: 933
  •  Gene Symbol: CD22
  •  SwissProt: P20273
  •  Chromosome Location: 19q13.1
  •  Unigene: 579691
NBT00974