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Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone L26 + IGEL/773]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Culture Supernatant
Culture Supernatant

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Culture Supernatant
Culture Supernatant

Price (USD)

199.00
429
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Monoclonal Antibody (L26 + IGEL/773)

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Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)

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Flow Cytometric Analysis of Raji cells using CD20 Monoclonal Antibody (L26 + IGEL/773) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26 + IGEL/773) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Product Details

Synonyms

APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Positive Control

Daudi, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (5-10ul/million cells)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human tonsil B cells (L26); Recombinant human MS4A1 protein (IGEL/773)

Cellular Localization

Predominantly cell surface with some cytoplasmic

Species Reactivity

Human. Baboon. Monkey. Does not react with Cow, Dog, Pig or Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa + Mouse / IgG2a, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

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Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Key References

  1. Schlossman, S., et al., eds. 1995. Leucocyte Typing V. New York: Oxford University Press.

Bioinformatics

  •  Entrez Gene ID: 931
  •  Gene Symbol: MS4A1
  •  SwissProt: P11836
  •  Chromosome Location: 11q12.2
  •  Unigene: 712553
NBT00854