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Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone B9E9]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab Conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab Conjugated to PE

Price (USD)

429.00

Product Details

Synonyms

APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Positive Control

Human lymphocytes or Raji cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry: 5ul per test per one million cells (or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Immunogen

Lymphoblastoid cell line Daudi

Cellular Localization

Predominantly cell surface with some cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Tedder TF and Schlossman SF. Phosphorylation of the B1 (CD20) molecule by normal and malignant human B lymphocytes. J Biol Chem 1988, 263(20):10009-10015.Bubien JK et al. Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes. J Cell Biol 1993, 121(5):1121-1132.Tedder TF and Engel P. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol Today 1994, 15(9):450-454.
  2. Schlossman S, et al. (eds). Leukocyte Typing V, Oxford University Press, Oxford, p511-515, 1995.
  3. Kanzaki M et al. Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells. J Biol Chem 1995, 270(22):13099-13104.

Bioinformatics

  •  Entrez Gene ID: 931
  •  Gene Symbol: MS4A1
  •  SwissProt: P11836
  •  Chromosome Location: 11q12.2
  •  Unigene: 712553
NBT00160